Primary infection with polyomavirus BK (BKV) in early childhood is usually asymptomatic. The virus establishes lifelong latency in cells of the urinary tract and kidneys. In immunosuppressed patients (e. g. after organ transplantation) however, virus reactivation may lead to severe complications and even renal failure. Close monitoring of BK viral load is of utmost importance in order to identify and evaluate potential virus reactivation at an early stage. Suitable therapeutic measures can thus be met before complications and irreversible organ damage occur. To date, the only known treatment is a controlled reduction in immunosuppression, which in turn has to be closely monitored, as it bears the risk of organ rejection. Therefore, a general BKV screening in regular intervals is advised after transplantation or complications.
FluoroType® BKV for fast and reliable quantification of BKV DNA from EDTA plasma or urine. Automated process steps minimize hands-on time for an efficient workflow. Viral DNA is extracted automatically with the GenoXtract®instrument followed by amplification, detection and quantification of characteristic target sequences with real-time PCR using the FluoroCycler®96. Internal controls monitor test performance from sample preparation to test result. Test results are indicated directly in International Units (IU/ml according to NIBSC WHO International Standard), no additional calculation or conversion has to be performed by the user. Reliable BK viral load assessment requires only two quantification standards, which are determined only once per kit lot, eliminating the need for standard measurements with each run. Additionally, virus concentrations beyond the linear quantification range are evaluated as qualitative results. Interpretation of results is performed automatically by the intuitive and user-friendly Fluoro-Software®, for reliable test results within only three hours. FluoroType® BKV enables reliable viral load assessment, ensuring close patient monitoring.