NUCLISENS EASYQ is the first automated system to combine Nucleic Acid Sequence-Based-Amplification (NASBA) amplification and real-time detection using molecular beacon probes for reliability and sensitivity. Designed for smooth, easy workflow, the system features intuitive operator software and short turnaround times for assays. It delivers the benefits of real-time moleculartechnology with minimized cross contamination risk.
What’s behind the NUCLISENS® EASYQ® instrument’s reliable and sensitive results? Nucleic Acid Sequence-Based-Amplification (NASBA) technology gives you accurate, real-time detection using molecular beacons. This specific iso-thermal method of nucleic acid amplification is a PCR alternative, and is highly adapted for the amplification of RNA and DNA.
NUCLISENS® EASYQ® is stress-less technology for streamlined workflow, thanks to intuitive software and short assay turnaround times - run times can be as low as 60 minutes (e.g. HIV viral load test). With real-time detection, its test format significantly reduces technical hands-on time and provides rapid results. What’s more, the isothermal NASBA reaction allows amplification runs with multiple targets. It all adds up to meeting the needs of the routine clinical laboratory for research applications and nucleic acid testing.
With a small footprint (42 x 42 cm), NUCLISENS® EASYQ® flexibly adapts to the needs of the different types and sizes of laboratories. And intuitive and straightforward software ensures uncomplicated daily use. You can adjust the testing to suit you. For example, it allows the use of commercial assays and “home-brew” assays developed through the NUCLISENS® EASYQ® Basic Kit.
Please consult your local bioMérieux representative for product availability in your country
NUCLISENS® assays are NASBA based nucleic acid amplification assays for the quantitative or qualitative detection of RNA (or adapted to DNA) in human samples. The NASBA amplification is based on primer extension and transcription by coordinated activities of three enzymes. The amplification reaction is isothermal and takes place at 41°C. Due to this relatively low temperature contaminating DNA cannot be amplified making the NASBA amplification specific for RNA amplification, even in a high DNA background.
Real-time detection using molecular beacons is based on the measurement of the time-related increase of a fluorescent signal. This signal is produced only after specific binding of the molecular beacon to an amplicon. The measurement is performed in combination with the amplification step. Results and interpretation of these signals is done by the NUCLISENS® EASYQ® Analyzer.
The NUCLISENS® EASYQ® Analyzer is dedicated to read fluorescence from reaction tubes in a controlled environment. The analyzer contains a plate carrier that keeps the samples at the required temperature. The fluorescent reader uses an advanced optical system, based on direct and focused illumination that prevents crosstalk, to give accurate readings. Filters control the wavelength of the illumination for both excitation and emission of the molecular beacon used in the assay. Filters are automatically selected by the assay protocol. The system uses different filters to measure particular labels (dyes).